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ppp1cb (a13528)  (ABclonal Biotechnology)

 
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    ABclonal Biotechnology ppp1cb (a13528)
    AFF4 disruption leads to increased CSTF2 occupancy with a 5′ shift. ( A ) The protein levels of CSTF2, CPSF3, SYMPK, XRN2, <t>PPP1CB,</t> and PPP1CC in WT, AFF4 KO, and AFF4 MT HCT-116 cells. α-Tubulin was used as a loading control. ( B ) Metaplot analysis of CSTF2 occupancies at the serum-inducible genes in WT, AFF4 KO, and AFF4 MT HCT-116 cells. CSTF2 densities are plotted in the region from −3 kb of the TSS to +3 kb of the TES within the genes. The metaplot was generated by rep1 ChIP–seq data that were verified by ChIP–qPCR. ( C ) Genome browser tracks of CSTF2 ChIP–seq at the serum-inducible genes FOS and EGR1 in WT, AFF4 KO, and AFF4 MT cells. Purple vertical dotted lines denote the TES and black arrows indicate Pol II peak shift toward 5′ ends. ( D and E ) RT–qPCR analysis of CSTF2 ( D ) and FOS ( E ) gene expression levels after CSTF2 knockdown in WT and AFF4 MT HCT-116 cells. ( F ) ChIP–qPCR analysis of Pol II occupancy at the FOS gene after CSTF2 knockdown in WT and AFF4 MT cells. The HEMO gene acts as a negative control for ChIP–qPCR. Data represent mean ± SEM of three biological replicates. ** P < 0.01, *** P < 0.001, **** P < 0.0001, t -test.
    Ppp1cb (A13528), supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ppp1cb (a13528)/product/ABclonal Biotechnology
    Average 90 stars, based on 1 article reviews
    ppp1cb (a13528) - by Bioz Stars, 2026-02
    90/100 stars

    Images

    1) Product Images from "Distinct roles of two SEC scaffold proteins, AFF1 and AFF4, in regulating RNA polymerase II transcription elongation"

    Article Title: Distinct roles of two SEC scaffold proteins, AFF1 and AFF4, in regulating RNA polymerase II transcription elongation

    Journal: Journal of Molecular Cell Biology

    doi: 10.1093/jmcb/mjad049

    AFF4 disruption leads to increased CSTF2 occupancy with a 5′ shift. ( A ) The protein levels of CSTF2, CPSF3, SYMPK, XRN2, PPP1CB, and PPP1CC in WT, AFF4 KO, and AFF4 MT HCT-116 cells. α-Tubulin was used as a loading control. ( B ) Metaplot analysis of CSTF2 occupancies at the serum-inducible genes in WT, AFF4 KO, and AFF4 MT HCT-116 cells. CSTF2 densities are plotted in the region from −3 kb of the TSS to +3 kb of the TES within the genes. The metaplot was generated by rep1 ChIP–seq data that were verified by ChIP–qPCR. ( C ) Genome browser tracks of CSTF2 ChIP–seq at the serum-inducible genes FOS and EGR1 in WT, AFF4 KO, and AFF4 MT cells. Purple vertical dotted lines denote the TES and black arrows indicate Pol II peak shift toward 5′ ends. ( D and E ) RT–qPCR analysis of CSTF2 ( D ) and FOS ( E ) gene expression levels after CSTF2 knockdown in WT and AFF4 MT HCT-116 cells. ( F ) ChIP–qPCR analysis of Pol II occupancy at the FOS gene after CSTF2 knockdown in WT and AFF4 MT cells. The HEMO gene acts as a negative control for ChIP–qPCR. Data represent mean ± SEM of three biological replicates. ** P < 0.01, *** P < 0.001, **** P < 0.0001, t -test.
    Figure Legend Snippet: AFF4 disruption leads to increased CSTF2 occupancy with a 5′ shift. ( A ) The protein levels of CSTF2, CPSF3, SYMPK, XRN2, PPP1CB, and PPP1CC in WT, AFF4 KO, and AFF4 MT HCT-116 cells. α-Tubulin was used as a loading control. ( B ) Metaplot analysis of CSTF2 occupancies at the serum-inducible genes in WT, AFF4 KO, and AFF4 MT HCT-116 cells. CSTF2 densities are plotted in the region from −3 kb of the TSS to +3 kb of the TES within the genes. The metaplot was generated by rep1 ChIP–seq data that were verified by ChIP–qPCR. ( C ) Genome browser tracks of CSTF2 ChIP–seq at the serum-inducible genes FOS and EGR1 in WT, AFF4 KO, and AFF4 MT cells. Purple vertical dotted lines denote the TES and black arrows indicate Pol II peak shift toward 5′ ends. ( D and E ) RT–qPCR analysis of CSTF2 ( D ) and FOS ( E ) gene expression levels after CSTF2 knockdown in WT and AFF4 MT HCT-116 cells. ( F ) ChIP–qPCR analysis of Pol II occupancy at the FOS gene after CSTF2 knockdown in WT and AFF4 MT cells. The HEMO gene acts as a negative control for ChIP–qPCR. Data represent mean ± SEM of three biological replicates. ** P < 0.01, *** P < 0.001, **** P < 0.0001, t -test.

    Techniques Used: Disruption, Generated, ChIP-sequencing, Quantitative RT-PCR, Expressing, Negative Control



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    ppp1cb (a13528)  (ABclonal Biotechnology)
    90
    ABclonal Biotechnology ppp1cb (a13528)
    AFF4 disruption leads to increased CSTF2 occupancy with a 5′ shift. ( A ) The protein levels of CSTF2, CPSF3, SYMPK, XRN2, <t>PPP1CB,</t> and PPP1CC in WT, AFF4 KO, and AFF4 MT HCT-116 cells. α-Tubulin was used as a loading control. ( B ) Metaplot analysis of CSTF2 occupancies at the serum-inducible genes in WT, AFF4 KO, and AFF4 MT HCT-116 cells. CSTF2 densities are plotted in the region from −3 kb of the TSS to +3 kb of the TES within the genes. The metaplot was generated by rep1 ChIP–seq data that were verified by ChIP–qPCR. ( C ) Genome browser tracks of CSTF2 ChIP–seq at the serum-inducible genes FOS and EGR1 in WT, AFF4 KO, and AFF4 MT cells. Purple vertical dotted lines denote the TES and black arrows indicate Pol II peak shift toward 5′ ends. ( D and E ) RT–qPCR analysis of CSTF2 ( D ) and FOS ( E ) gene expression levels after CSTF2 knockdown in WT and AFF4 MT HCT-116 cells. ( F ) ChIP–qPCR analysis of Pol II occupancy at the FOS gene after CSTF2 knockdown in WT and AFF4 MT cells. The HEMO gene acts as a negative control for ChIP–qPCR. Data represent mean ± SEM of three biological replicates. ** P < 0.01, *** P < 0.001, **** P < 0.0001, t -test.
    Ppp1cb (A13528), supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ppp1cb (a13528)/product/ABclonal Biotechnology
    Average 90 stars, based on 1 article reviews
    ppp1cb (a13528) - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier
    ppp1cb (a13528  (ABclonal Biotechnology)
    90
    ABclonal Biotechnology ppp1cb (a13528
    (A) The protein levels of CPF components CSTF2, CPSF3, SYMPK, Xrn2, <t>PPP1CB</t> and PPP1CC in AFF4 depletion and AFF4 mutation HCT116 cells. α-Tubulin was used as a loading control. (B) Schematic diagram shows the primers sites around FOS gene. ChIP-qPCR analysis showing the occupancy of CSTF2 at FOS gene in AFF4 depletion and AFF4 mutation serum induced HCT116 cells. The HEMO gene acts as a negative control for ChIP-qPCR. (C) RT-qPCR analysis of CSTF2 gene expression after CSTF2 knockdown in wildtype and AFF4 mutation HCT116 cell lines. (D) RT-qPCR analysis of FOS gene expression after CSTF2 knockdown in wildtype and AFF4 mutation HCT116 cell lines. (E) ChIP-qPCR showing the occupancy of Pol II after CSTF2 knockdown in AFF4 mut. The HEMO gene acts as a negative control for ChIP-qPCR.
    Ppp1cb (A13528, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ppp1cb (a13528/product/ABclonal Biotechnology
    Average 90 stars, based on 1 article reviews
    ppp1cb (a13528 - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    AFF4 disruption leads to increased CSTF2 occupancy with a 5′ shift. ( A ) The protein levels of CSTF2, CPSF3, SYMPK, XRN2, PPP1CB, and PPP1CC in WT, AFF4 KO, and AFF4 MT HCT-116 cells. α-Tubulin was used as a loading control. ( B ) Metaplot analysis of CSTF2 occupancies at the serum-inducible genes in WT, AFF4 KO, and AFF4 MT HCT-116 cells. CSTF2 densities are plotted in the region from −3 kb of the TSS to +3 kb of the TES within the genes. The metaplot was generated by rep1 ChIP–seq data that were verified by ChIP–qPCR. ( C ) Genome browser tracks of CSTF2 ChIP–seq at the serum-inducible genes FOS and EGR1 in WT, AFF4 KO, and AFF4 MT cells. Purple vertical dotted lines denote the TES and black arrows indicate Pol II peak shift toward 5′ ends. ( D and E ) RT–qPCR analysis of CSTF2 ( D ) and FOS ( E ) gene expression levels after CSTF2 knockdown in WT and AFF4 MT HCT-116 cells. ( F ) ChIP–qPCR analysis of Pol II occupancy at the FOS gene after CSTF2 knockdown in WT and AFF4 MT cells. The HEMO gene acts as a negative control for ChIP–qPCR. Data represent mean ± SEM of three biological replicates. ** P < 0.01, *** P < 0.001, **** P < 0.0001, t -test.

    Journal: Journal of Molecular Cell Biology

    Article Title: Distinct roles of two SEC scaffold proteins, AFF1 and AFF4, in regulating RNA polymerase II transcription elongation

    doi: 10.1093/jmcb/mjad049

    Figure Lengend Snippet: AFF4 disruption leads to increased CSTF2 occupancy with a 5′ shift. ( A ) The protein levels of CSTF2, CPSF3, SYMPK, XRN2, PPP1CB, and PPP1CC in WT, AFF4 KO, and AFF4 MT HCT-116 cells. α-Tubulin was used as a loading control. ( B ) Metaplot analysis of CSTF2 occupancies at the serum-inducible genes in WT, AFF4 KO, and AFF4 MT HCT-116 cells. CSTF2 densities are plotted in the region from −3 kb of the TSS to +3 kb of the TES within the genes. The metaplot was generated by rep1 ChIP–seq data that were verified by ChIP–qPCR. ( C ) Genome browser tracks of CSTF2 ChIP–seq at the serum-inducible genes FOS and EGR1 in WT, AFF4 KO, and AFF4 MT cells. Purple vertical dotted lines denote the TES and black arrows indicate Pol II peak shift toward 5′ ends. ( D and E ) RT–qPCR analysis of CSTF2 ( D ) and FOS ( E ) gene expression levels after CSTF2 knockdown in WT and AFF4 MT HCT-116 cells. ( F ) ChIP–qPCR analysis of Pol II occupancy at the FOS gene after CSTF2 knockdown in WT and AFF4 MT cells. The HEMO gene acts as a negative control for ChIP–qPCR. Data represent mean ± SEM of three biological replicates. ** P < 0.01, *** P < 0.001, **** P < 0.0001, t -test.

    Article Snippet: Pol II Ser2P (ab5095), Pol II Ser5P (ab5131), and PAF1 (ab20662) rabbit polyclonal antibodies were purchased from Abcam; SPT5 (A9193), SPT6 (A16434), CSTF2 (A8116), CPSF3 (A12368), SYMPK (A8722), XRN2 (A18350), PPP1CB (A13528), and PPP1CC (A4025) rabbit polyclonal antibodies were purchased from ABclonal.

    Techniques: Disruption, Generated, ChIP-sequencing, Quantitative RT-PCR, Expressing, Negative Control

    (A) The protein levels of CPF components CSTF2, CPSF3, SYMPK, Xrn2, PPP1CB and PPP1CC in AFF4 depletion and AFF4 mutation HCT116 cells. α-Tubulin was used as a loading control. (B) Schematic diagram shows the primers sites around FOS gene. ChIP-qPCR analysis showing the occupancy of CSTF2 at FOS gene in AFF4 depletion and AFF4 mutation serum induced HCT116 cells. The HEMO gene acts as a negative control for ChIP-qPCR. (C) RT-qPCR analysis of CSTF2 gene expression after CSTF2 knockdown in wildtype and AFF4 mutation HCT116 cell lines. (D) RT-qPCR analysis of FOS gene expression after CSTF2 knockdown in wildtype and AFF4 mutation HCT116 cell lines. (E) ChIP-qPCR showing the occupancy of Pol II after CSTF2 knockdown in AFF4 mut. The HEMO gene acts as a negative control for ChIP-qPCR.

    Journal: bioRxiv

    Article Title: Distinct roles of the two SEC scaffold proteins, AFF1 and AFF4, in regulating RNA Pol II transcription elongation

    doi: 10.1101/2022.08.09.503397

    Figure Lengend Snippet: (A) The protein levels of CPF components CSTF2, CPSF3, SYMPK, Xrn2, PPP1CB and PPP1CC in AFF4 depletion and AFF4 mutation HCT116 cells. α-Tubulin was used as a loading control. (B) Schematic diagram shows the primers sites around FOS gene. ChIP-qPCR analysis showing the occupancy of CSTF2 at FOS gene in AFF4 depletion and AFF4 mutation serum induced HCT116 cells. The HEMO gene acts as a negative control for ChIP-qPCR. (C) RT-qPCR analysis of CSTF2 gene expression after CSTF2 knockdown in wildtype and AFF4 mutation HCT116 cell lines. (D) RT-qPCR analysis of FOS gene expression after CSTF2 knockdown in wildtype and AFF4 mutation HCT116 cell lines. (E) ChIP-qPCR showing the occupancy of Pol II after CSTF2 knockdown in AFF4 mut. The HEMO gene acts as a negative control for ChIP-qPCR.

    Article Snippet: Ser2P Pol II (ab5095), Ser5P Pol II (ab5131), PAF1 (ab20662) rabbit polyclonal antibody were purchased from Abcam; SPT5 (A9193), SPT6 (A16434), CSTF2 (A8116), CPSF3 (A12368), SYMPK (A8722), Xrn2 (A18350), PPP1CB (A13528), PPP1CC (A4025) rabbit polyclonal antibody were purchased from ABclonal.

    Techniques: Mutagenesis, Negative Control, Quantitative RT-PCR, Expressing

    (A) The protein levels of CPF components CSTF2, CPSF3, SYMPK, Xrn2, PPP1CB and PPP1CC in AFF4 depletion and AFF4 mutation serum induced HCT116 cells. α-Tubulin was used as a loading control. (B) RT-qPCR showing the RNA expression level of CPSF3 after CPSF3 knockdown in wildtype and AFF4 MT cells. (C) RT-qPCR showing the RNA expression level of FOS after CPSF3 knockdown in wildtype and AFF4 MT cells. (D) The change of Pol II enrichment around the FOS gene after knockdown CPSF3 in WT and AFF4 MT cells analyzed by ChIP-qPCR. The HEMO gene acts as a negative control for ChIP-qPCR.

    Journal: bioRxiv

    Article Title: Distinct roles of the two SEC scaffold proteins, AFF1 and AFF4, in regulating RNA Pol II transcription elongation

    doi: 10.1101/2022.08.09.503397

    Figure Lengend Snippet: (A) The protein levels of CPF components CSTF2, CPSF3, SYMPK, Xrn2, PPP1CB and PPP1CC in AFF4 depletion and AFF4 mutation serum induced HCT116 cells. α-Tubulin was used as a loading control. (B) RT-qPCR showing the RNA expression level of CPSF3 after CPSF3 knockdown in wildtype and AFF4 MT cells. (C) RT-qPCR showing the RNA expression level of FOS after CPSF3 knockdown in wildtype and AFF4 MT cells. (D) The change of Pol II enrichment around the FOS gene after knockdown CPSF3 in WT and AFF4 MT cells analyzed by ChIP-qPCR. The HEMO gene acts as a negative control for ChIP-qPCR.

    Article Snippet: Ser2P Pol II (ab5095), Ser5P Pol II (ab5131), PAF1 (ab20662) rabbit polyclonal antibody were purchased from Abcam; SPT5 (A9193), SPT6 (A16434), CSTF2 (A8116), CPSF3 (A12368), SYMPK (A8722), Xrn2 (A18350), PPP1CB (A13528), PPP1CC (A4025) rabbit polyclonal antibody were purchased from ABclonal.

    Techniques: Mutagenesis, Quantitative RT-PCR, RNA Expression, Negative Control